This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Reprints and Permissions Copyright Information Books from ASM Press MicrobeWorld Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Lally, N. C. Articles by Dubey, J. P. Search for Related Content PubMed PubMed Citation Articles by Lally, N. C. Articles by Dubey, J. P.

 Previous Article  |  Next Article 

Clinical and Diagnostic Laboratory Immunology, May 1996, 275-279, Vol 3, No. 3
Copyright © 1996 by the American Society for Microbiology. All rights reserved.

Evaluation of two Neospora caninum recombinant antigens for use in an enzyme-linked immunosorbent assay for the diagnosis of bovine neosporosis

NC Lally, MC Jenkins and JP Dubey
Parasite Immunobiology Laboratory, USDA Agricultural Research Service, Beltsville, Maryland 20705, USA.

Neospora caninum is a recently described apicomplexan parasite which causes paralysis and death in dogs. Neospora parasites also cause abortion and neonatal morbidity in cattle, sheep, goats, and horses, and neosporosis is emerging as an important cause of bovine abortion worldwide. The purpose of this study was to identify N. caninum cDNA clones encoding antigens that would be useful for the immunodiagnosis of bovine neosporosis. Two N. caninum tachyzoite cDNA clones expressing antigens that were recognized by serum from naturally and experimentally infected cattle were identified. The DNA sequences of these clones were determined, and the inserts were subcloned into the plasmid expression vector pTrcHisB. Both recombinant antigens, expressed as fusion proteins with a His6 tag, were purified on a nickel- chelating affinity column and evaluated in separate enzyme-linked immunosorbent assays (ELISAs). Both recombinant antigen ELISAs were capable of distinguishing between sera from Neospora-infected cows and sera from uninfected control cows. Furthermore, both assays were able to detect an antibody response in animals that were experimentally inoculated with N. caninum. Neither antigen showed evidence of cross- reactivity with serum from animals inoculated with the closely related parasites Toxoplasma gondii, Sarcocystis cruzi, Sarcocystis hominis, and Sarcocystis hirsuta.

This article has been cited by other articles:

• Nishikawa, Y., Kousaka, Y., Tragoolpua, K., Xuan, X., Makala, L., Fujisaki, K., Mikami, T., Nagasawa, H. (2001). Characterization of Neospora caninum Surface Protein NcSRS2 Based on Baculovirus Expression System and Its Application for Serodiagnosis of Neospora Infection. J. Clin. Microbiol. 39: 3987-3991 [Abstract] [Full Text]  
• Wouda, W., Brinkhof, J., van Maanen, C., de Gee, A. L. W., Moen, A. R. (1998). Serodiagnosis of Neosporosis in Individual Cows and Dairy Herds: A Comparative Study of Three Enzyme-Linked Immunosorbent Assays. CVI 5: 711-716 [Abstract] [Full Text]  
• Packham, A. E., Sverlow, K. W., Conrad, P. A., Loomis, E. F., Rowe, J. D., Anderson, M. L., Marsh, A. E., Cray, C., Barr, B. C. (1998). A Modified Agglutination Test for Neospora caninum: Development, Optimization, and Comparison to the Indirect Fluorescent-Antibody Test and Enzyme-Linked Immunosorbent Assay. CVI 5: 467-473 [Abstract] [Full Text]