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Clinical and Diagnostic Laboratory Immunology, 03 1996, 242-245, Vol 3, No. 2
Copyright © 1996 by the American Society for Microbiology. All rights reserved.

Antibody response of monkeys to invasion plasmid antigen D after infection with Shigella spp

EV Oaks, WD Picking and WL Picking
Department of Enteric Infections, Walter Reed Army Institute of Research, Washington, D.C. 20307, USA.

The antigen preparation most often used for determining the levels of antibodies to virulence-associated proteins of Shigella spp. consists of a mixture of proteins (including IpaB, IpaC, IpaD, and VirG*) extracted from virulent shigellae with water (water extract). To overcome the lack of specificity for individual antigens in the water- extract enzyme-linked immunosorbent assay (ELISA), the ipaD gene from S. flexneri has been cloned, expressed to a high level, and purified for use in a new ELISA for the determination of the levels of antibody against IpaD in monkeys and humans challenged with shigellae. The IpaD ELISA for serum immunoglobulins G and A correlated well with the water- extract ELISA in that monkeys infected with S. flexneri or S. sonnei responded with high serum antibody titers in both assays. The IpaD assay required less antigen per well, had much lower background levels, and did not require correction with antigens from an avirulent organism. In conjunction with the water-extract ELISA, it was possible to identify infected animals that did not respond to IpaD but did produce antibodies that reacted in the water-extract ELISA. This indicates that even though IpaB, IpaC, and IpaD are essential for the invasiveness phenotype, the infected host does not always produce antibodies against all components of the invasiveness apparatus.

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• Kadurugamuwa, J. L., Beveridge, T. J. (1998). Delivery of the Non-Membrane-Permeative Antibiotic Gentamicin into Mammalian Cells by Using Shigella flexneri Membrane Vesicles. Antimicrob. Agents Chemother. 42: 1476-1483 [Abstract] [Full Text]  
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