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Clinical and Diagnostic Laboratory Immunology, Mar 1996, 239-241, Vol 3, No. 2
Copyright © 1996 by the American Society for Microbiology. All rights reserved.

Immunological characterization of a recombinant 27-kilodalton antigenic protein from Paracoccidioides brasiliensis

BL Ortiz, AM Garcia, A Restrepo and JG McEwen
Molecular Biology Section, Corporacion para Investigaciones Biologicas, Medellin, Colombia.

We report the expression in Escherichia coli of a 27-kDa antigenic protein from Paracoccidioides brasiliensis. When analyzed by immunoblotting, this recombinant antigenic protein was recognized by antibodies present in the sera of 40 of the 44 paracoccidioidomycosis patients studied. No cross-reactions were observed with sera from patients with other mycoses (histoplasmosis, aspergillosis, cryptococcosis, sporotrichosis, and chromoblastomycosis) or with tuberculosis.

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