This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Reprints and Permissions Copyright Information Books from ASM Press MicrobeWorld Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Tolfvenstam, T. Articles by Broliden, K. Search for Related Content PubMed PubMed Citation Articles by Tolfvenstam, T. Articles by Broliden, K.

 Previous Article  |  Next Article 

Clinical and Diagnostic Laboratory Immunology, Mar 1996, 147-150, Vol 3, No. 2
Copyright © 1996 by the American Society for Microbiology. All rights reserved.

Evaluation of serological assays for identification of parvovirus B19 immunoglobulin M

T Tolfvenstam, U Ruden and K Broliden
Swedish Institute for Infectious Disease Control, Stockholm.

Three different enzyme immunoassays (EIAs) (Parvoscan-B19, IBL parvovirus B19, and IDEIA parvovirus B19) and one immunofluorescence assay (Biotrin Parvo B19 IFA) were evaluated for detection of parvovirus B19 immunoglobulin M (IgM) antibodies in 203 clinical serum samples. An IgM antibody capture radioimmunoassay was used as a reference test. Serum specimens obtained from patients with clinical symptoms suggestive of parvovirus B19 infections were used to evaluate the sensitivities of the assays, which were shown to be comparable for the Biotrin IFA and IDEIA (97%) and lower for the other two EIAs (90%). In order to test the specificity of the assays, clinical serum samples with IgM antibodies against other viruses were examined, as well as sera with rheumatoid factor activity and sera from healthy pregnant women. The specificities of B19 IgM antibody detection were 96% for the Biotrin IFA, 96% for IDEIA, 90% for Parvoscan, and 88% for the IBL assay. These results show that all four assays can be recommended for diagnostic purposes, although false-positive results may be seen with other acute viral infections, healthy pregnant women, and rheumatoid factor-positive samples.

This article has been cited by other articles:

• Corcoran, A., Doyle, S. (2004). Advances in the biology, diagnosis and host-pathogen interactions of parvovirus B19. J Med Microbiol 53: 459-475 [Abstract] [Full Text]  
• Noyola, D. E., Padilla-Ruiz, M. L., Obregon-Ramos, M. G., Zayas, P., Perez-Romano, B. (2004). Parvovirus B19 infection in medical students during a hospital outbreak. J Med Microbiol 53: 141-146 [Abstract] [Full Text]