This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Reprints and Permissions Copyright Information Books from ASM Press MicrobeWorld Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Kumar, V. Articles by Wortsman, J. Search for Related Content PubMed PubMed Citation Articles by Kumar, V. Articles by Wortsman, J.

 Previous Article  |  Next Article 

Clinical and Diagnostic Laboratory Immunology, 03 1996, 143-146, Vol 3, No. 2
Copyright © 1996 by the American Society for Microbiology. All rights reserved.

Celiac disease and hypoparathyroidism: cross-reaction of endomysial antibodies with parathyroid tissue

V Kumar, JE Valeski and J Wortsman
IMMCO Diagnostics, Inc., Buffalo, NY 14223, USA.

Celiac disease (CD) is a gluten-sensitive enteropathy characterized by the presence of serum antibodies to endomysial reticulin and gliadin antigens. CD has been associated with various autoimmune endocrine disorders, such as diabetes. We report a rare case of idiopathic hypoparathyroidism with coexistent CD characterized by the presence of serum autoantibodies. Studies were conducted to determine the specificities of these autoantibodies and to localize the antibody binding sites by indirect immunofluorescence and immunoelectron microscopy. Sera from a patient with idiopathic hypoparathyroidism and CD and from two patients with CD alone were tested by indirect immunofluorescence for autoantibodies to parathyroid and endomysial antigens. The specificities of the antibody reactions were determined by testing the sera before and after absorption with monkey stomach tissue. In addition, immunoelectron microscopic studies were performed to determine the localization of the endomysial antigen. Indirect- immunofluorescence studies on the patient's serum were positive with the parathyroid as well as the endomysial substrate. Similar reactions were also observed with the sera of endomysial antibody-positive patients with CD. Absorption of the sera with monkey stomach powder, which is known to have the endomysial antigen, abolished the antibody activities on both the endomysial substrate and the parathyroid tissue. Immunoelectron microscopic studies showed that endomysial antibody activity was associated with antigens localized on the myocyte plasma membrane and in the intercellular spaces. Thus, reactions of the patient's serum with the parathyroid tissue were due to endomysial antibodies and were not parathyroid specific as in patients with idiopathic hypoparathyroidism who did not have coexistent CD. In conclusion, indirect-immunofluorescence tests on parathyroid tissue detect not only tissue-specific antibodies but also cross-reactive antibodies, and this should be taken into consideration when these tests are performed.

This article has been cited by other articles:

• Collin, P., Kaukinen, K., Valimaki, M., Salmi, J. (2002). Endocrinological Disorders and Celiac Disease. Endocr. Rev. 23: 464-483 [Abstract] [Full Text]  
• Kumar, V., Rajadhyaksha, M., Wortsman, J. (2001). Celiac Disease-Associated Autoimmune Endocrinopathies. CVI 8: 678-685 [Abstract] [Full Text]