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Clinical and Diagnostic Laboratory Immunology, 11 1995, 770-775, Vol 2, No. 6
Copyright © 1995 by the American Society for Microbiology. All rights reserved.

Comparison of PCR-generated fragments of the mce gene from Mycobacterium tuberculosis, M. avium, M. intracellulare, and M. scrofulaceum

SL Parker, YL Tsai and CJ Palmer
Environmental Sciences Laboratory, County Sanitation Districts of Orange County, Fountain Valley, California 92728-8127, USA.

Nineteen strains representing 13 species of mycobacteria were tested for the ability to serve as PCR templates for the production of a 293- bp fragment of the mycobacterial mce gene. The mce gene is a virulence factor recently sequenced from Mycobacterium tuberculosis. PCR products were obtained for only the species of the Mycobacterium tuberculosis complex (MTC) and the Mycobacterium avium-M. intracellulare-M. scrofulaceum complex. The fragment was sequenced from M. tuberculosis (one strain), M. avium (three strains), M. intracellulare (two strains), and M. scrofulaceum (two strains). Sequence comparisons suggest that the fragments from each of the species are regions that code for a similar product. One of the M. scrofulaceum strains yielded a sequence whose most probable reading frame was truncated by an amber stop codon caused by a single nuclei acid difference from the other sequences. The amino acid sequences from the non-MTC sequences cluster together, displaying fewer differences from each other than from the M. tuberculosis sequence and the truncated M. scrofulaceum sequence. Principal component analysis of the distance matrix displays the clustering of the M. avium and M. intracellulare sequences into single- species clusters. It is concluded that at least one open reading frame of the mce gene is found, although it is discernibly different, in pathogenic mycobacteria other than the MTC.

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