This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Reprints and Permissions Copyright Information Books from ASM Press MicrobeWorld Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Raeder, R. Articles by Boyle, M. D. Search for Related Content PubMed PubMed Citation Articles by Raeder, R. Articles by Boyle, M. D.

 Previous Article  |  Next Article 

Clinical and Diagnostic Laboratory Immunology, Jul 1995, 478-483, Vol 2, No. 4
Copyright © 1995 by the American Society for Microbiology. All rights reserved.

Distinct profiles of immunoglobulin G-binding-protein expression by invasive serotype M1 isolates of Streptococcus pyogenes

R Raeder and MD Boyle
Department of Microbiology, Medical College of Ohio, Toledo 43699-0008, USA.

Analysis of immunoglobulin G (IgG)-binding-protein expression by invasive group A streptococcal isolates of the M1 serotype collected as part of a Centers for Disease Control and Prevention surveillance study revealed two distinct phenotypes. One group of type M1 isolates expressed a surface protein reactive with all four human IgG subclasses (type IIo), while a second group expressed a surface protein demonstrating significant reactivity only with human IgG3 (type IIb). The functional forms of IgG-binding protein were antigenically related, and both were recognized by a rabbit polyclonal antiserum to serotype M1 but not by normal rabbit serum. While the quantities of antigenic M1 protein present in the extracts of representative isolates displaying each phenotype differed, the functional differences were found to be qualitative and not solely quantitative. The IgG-binding properties of these antigenically related M1 proteins could be readily distinguished from those of another IgG-binding protein, protein H. Type M1 isolates of the IIb phenotype differed from those of the IIo phenotype by secreting larger amounts of a casein-hydrolyzing protease into culture supernatants.

This article has been cited by other articles:

• Rezcallah, M. S., Boyle, M. D. P., Sledjeski, D. D. (2004). Mouse skin passage of Streptococcus pyogenes results in increased streptokinase expression and activity. Microbiology 150: 365-371 [Abstract] [Full Text]  
• Fagan, P. K., Reinscheid, D., Gottschalk, B., Chhatwal, G. S. (2001). Identification and Characterization of a Novel Secreted Immunoglobulin Binding Protein from Group A Streptococcus. Infect. Immun. 69: 4851-4857 [Abstract] [Full Text]  
• Raeder, R., Harokopakis, E., Hollingshead, S., Boyle, M. D. P. (2000). Absence of SpeB Production in Virulent Large Capsular Forms of Group A Streptococcal Strain 64. Infect. Immun. 68: 744-751 [Abstract] [Full Text]  
• Li, Z., Sledjeski, D. D., Kreikemeyer, B., Podbielski, A., Boyle, M. D. P. (1999). Identification of pel, a Streptococcus pyogenes Locus That Affects both Surface and Secreted Proteins. J. Bacteriol. 181: 6019-6027 [Abstract] [Full Text]