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Clinical and Diagnostic Laboratory Immunology, 05 1995, 365-368, Vol 2, No. 3
Copyright © 1995 by the American Society for Microbiology. All rights reserved.

Assessment of complement-mediated killing of Moraxella (Branhamella) catarrhalis isolates by a simple method

CM Verduin, C Hol, E Van Dijke, JA Faber, M Jansze, J Verhoef and H Van Dijk
Eijkman-Winkler Institute for Medical and Clinical Microbiology, Faculty of Medicine, Utrecht University Hospital, The Netherlands.

Recently, we showed that complement resistance is an important virulence factor of Moraxella (Branhamella) catarrhalis. Our study used a serum bactericidal assay to determine complement resistance in M. catarrhalis. Although the serum bactericidal assay is considered the "gold standard" for determining complement resistance, it is laborious and time-consuming and therefore not well suited for large-scale studies. Using a large number (n = 324) of M. catarrhalis isolates obtained from the sputa of patients with lower respiratory tract infections (n = 200) and young carriers (n = 124), we assessed the value of a simple "culture-and-spot" test as an alternative to the serum bactericidal assay. For both groups of isolates, the degree of concordance between the two tests used was very significant (P < 0.0001). The agreement between the two assays was estimated to be "excellent beyond chance" (as determined by Cohen's kappa test). The culture-and-spot assay is a valuable alternative to the serum bactericidal assay, not only for screening purposes as shown here but also for studying the mechanism of complement resistance in M. catarrhalis at the molecular level.

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