This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Reprints and Permissions Copyright Information Books from ASM Press MicrobeWorld Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Hemmen, F. Articles by Saman, E. Search for Related Content PubMed PubMed Citation Articles by Hemmen, F. Articles by Saman, E.

 Previous Article  |  Next Article 

Clinical and Diagnostic Laboratory Immunology, 05 1995, 263-267, Vol 2, No. 3
Copyright © 1995 by the American Society for Microbiology. All rights reserved.

Cloning and sequence analysis of a newly identified Brucella abortus gene and serological evaluation of the 17-kilodalton antigen that it encodes

F Hemmen, V Weynants, T Scarcez, JJ Letesson and E Saman
Innogenetics N.V., Ghent, Belgium.

A thus far unknown gene encoding a Brucella abortus protein has been isolated from a lambda gt11 expression library probed with sera from Brucella-infected sheep. Sequence analysis of the cloned gene revealed the presence of an open reading frame of 158 amino acids encoding a protein of 17.3 kDa (calculated molecular mass). The recombinant B. abortus protein, expressed in Escherichia coli, and the corresponding Brucella melitensis protein migrated at the same apparent molecular masses as shown by Western blotting (immunoblotting). Among a series of serum samples from B. melitensis- or B. abortus-infected sheep and cows, 51 and 39%, respectively, showed a signal at 17 kDa on Western blot analysis of total protein extract from Brucella bacteria. These figures amount to 70 and 61% for sheep and cattle, respectively, in a competitive enzyme-linked immunosorbent assay with a specific monoclonal antibody. These data indicate that the 17-kDa antigen may be useful for serological diagnosis of Brucella infection.

This article has been cited by other articles:

• Ko, J., Splitter, G. A. (2003). Molecular Host-Pathogen Interaction in Brucellosis: Current Understanding and Future Approaches to Vaccine Development for Mice and Humans. Clin. Microbiol. Rev. 16: 65-78 [Abstract] [Full Text]  
• Velikovsky, C. A., Cassataro, J., Giambartolomei, G. H., Goldbaum, F. A., Estein, S., Bowden, R. A., Bruno, L., Fossati, C. A., Spitz, M. (2002). A DNA Vaccine Encoding Lumazine Synthase from Brucella abortus Induces Protective Immunity in BALB/c Mice. Infect. Immun. 70: 2507-2511 [Abstract] [Full Text]  
• Marin, C. M., Moreno, E., Moriyon, I., Diaz, R., Blasco, J. M. (1999). Performance of Competitive and Indirect Enzyme-Linked Immunosorbent Assays, Gel Immunoprecipitation with Native Hapten Polysaccharide, and Standard Serological Tests in Diagnosis of Sheep Brucellosis. CVI 6: 269-272 [Abstract] [Full Text]  
• Alonso-Urmeneta, B., Marín, C., Aragón, V., Blasco, J. M., Díaz, R., Moriyón, I. (1998). Evaluation of Lipopolysaccharides and Polysaccharides of Different Epitopic Structures in the Indirect Enzyme-Linked Immunosorbent Assay for Diagnosis of Brucellosis in Small Ruminants and Cattle. CVI 5: 749-754 [Abstract] [Full Text]  
• Cloeckaert, A., Weynants, V., Godfroid, J., Verger, J.-M., Grayon, M., Zygmunt, M. S. (1998). O-Polysaccharide Epitopic Heterogeneity at the Surface of Brucella spp. Studied by Enzyme-Linked Immunosorbent Assay and Flow Cytometry. CVI 5: 862-870 [Abstract] [Full Text]  
• Tibor, A., Jacques, I., Guilloteau, L., Verger, J.-M., Grayon, M., Wansard, V., Letesson, J.-J. (1998). Effect of P39 Gene Deletion in Live Brucella Vaccine Strains on Residual Virulence and Protective Activity in Mice. Infect. Immun. 66: 5561-5564 [Abstract] [Full Text]