This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Reprints and Permissions Copyright Information Books from ASM Press MicrobeWorld Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Theisen, M. Articles by Hogh, B. Search for Related Content PubMed PubMed Citation Articles by Theisen, M. Articles by Hogh, B.

 Previous Article  |  Next Article 

Clinical and Diagnostic Laboratory Immunology, Jan 1995, 30-34, Vol 2, No. 1
Copyright © 1995 by the American Society for Microbiology. All rights reserved.

Antigenicity and immunogenicity of recombinant glutamate-rich protein of Plasmodium falciparum expressed in Escherichia coli

M Theisen, J Vuust, A Gottschau, S Jepsen and B Hogh
Department of Infection-Immunology, Statens Seruminstitut, Copenhagen S, Denmark.

A recombinant Plasmodium falciparum glutamate-rich protein (GLURP) was produced in Escherichia coli as a nearly full-length protein. In order to map immunodominant regions on GLURP, the nonrepetitive amino- terminal region (R0) as well as the central repeat region (R1) and the carboxy-terminal repeat region (R2) were also produced as separate products. All four purified gene products reacted specifically with serum samples from adults living in an area of Liberia where malaria is holoendemic. It appears that the human immune response against GLURP is primarily directed against the R2 region because 94% of the serum samples reacted with this region in an immunoassay. Antibody reactivity against the R0 region was also observed in 75% of the serum samples, while the R1 region showed only weak antibody-binding activity. When the nearly full-length GLURP molecule was adsorbed to Al(OH)3 it was found to be immunogenic in mice. In these experiments, the antibody response was almost exclusively directed against the R2 region. When anti-GLURP sera were obtained from rabbits immunized with the three regions, R0, R1, and R2, respectively, they recognized in immunoprecipitation experiments authentic GLURP from P. falciparum grown in vitro. These results demonstrate that GLURP produced in E. coli can induce a humoral immune response against GLURP derived from blood-stage parasites.

This article has been cited by other articles:

• Brustoski, K., Kramer, M., Moller, U., Kremsner, P. G., Luty, A. J. F. (2005). Neonatal and Maternal Immunological Responses to Conserved Epitopes within the DBL-{gamma}3 Chondroitin Sulfate A-Binding Domain of Plasmodium falciparum Erythrocyte Membrane Protein 1. Infect. Immun. 73: 7988-7995 [Abstract] [Full Text]  
• PRATT-RICCIO, L. R., LIMA-JUNIOR, J. C., CARVALHO, L. J. M., THEISEN, M., ESPINDOLA-MENDES, E. C., SANTOS, F., OLIVEIRA-FERREIRA, J., GOLDBERG, A. C., DANIEL-RIBEIRO, C. T., BANIC, D. M. (2005). ANTIBODY RESPONSE PROFILES INDUCED BY PLASMODIUM FALCIPARUM GLUTAMATE-RICH PROTEIN IN NATURALLY EXPOSED INDIVIDUALS FROM A BRAZILIAN AREA ENDEMIC FOR MALARIA. Am J Trop Med Hyg 73: 1096-1103 [Abstract] [Full Text]  
• Rosenkrands, I., Agger, E. M., Olsen, A. W., Korsholm, K. S., Andersen, C. S., Jensen, K. T., Andersen, P. (2005). Cationic Liposomes Containing Mycobacterial Lipids: a New Powerful Th1 Adjuvant System. Infect. Immun. 73: 5817-5826 [Abstract] [Full Text]  
• Singh, S., Soe, S., Roussilhon, C., Corradin, G., Druilhe, P. (2005). Plasmodium falciparum Merozoite Surface Protein 6 Displays Multiple Targets for Naturally Occurring Antibodies That Mediate Monocyte-Dependent Parasite Killing. Infect. Immun. 73: 1235-1238 [Abstract] [Full Text]  
• Theisen, M., Dodoo, D., Toure-Balde, A., Soe, S., Corradin, G., Koram, K. K., Kurtzhals, J. A. L., Hviid, L., Theander, T., Akanmori, B., Ndiaye, M., Druilhe, P. (2001). Selection of Glutamate-Rich Protein Long Synthetic Peptides for Vaccine Development: Antigenicity and Relationship with Clinical Protection and Immunogenicity. Infect. Immun. 69: 5223-5229 [Abstract] [Full Text]  
• Oeuvray, C., Theisen, M., Rogier, C., Trape, J.-F., Jepsen, S., Druilhe, P. (2000). Cytophilic Immunoglobulin Responses to Plasmodium falciparum Glutamate-Rich Protein Are Correlated with Protection against Clinical Malaria in Dielmo, Senegal. Infect. Immun. 68: 2617-2620 [Abstract] [Full Text]  
• Skjot, R. L. V., Oettinger, T., Rosenkrands, I., Ravn, P., Brock, I., Jacobsen, S., Andersen, P. (2000). Comparative Evaluation of Low-Molecular-Mass Proteins from Mycobacterium tuberculosis Identifies Members of the ESAT-6 Family as Immunodominant T-Cell Antigens. Infect. Immun. 68: 214-220 [Abstract] [Full Text]  
• Mathiesen, M. J., Holm, A., Christiansen, M., Blom, J., Hansen, K., Ostergaard, S., Theisen, M. (1998). The Dominant Epitope of Borrelia garinii Outer Surface Protein C Recognized by Sera from Patients with Neuroborreliosis Has a Surface-Exposed Conserved Structural Motif. Infect. Immun. 66: 4073-4079 [Abstract] [Full Text]  
• Rosenkrands, I., Rasmussen, P. B., Carnio, M., Jacobsen, S., Theisen, M., Andersen, P. (1998). Identification and Characterization of a 29-Kilodalton Protein from Mycobacterium tuberculosis Culture Filtrate Recognized by Mouse Memory Effector Cells. Infect. Immun. 66: 2728-2735 [Abstract] [Full Text]  
• Harboe, M., Malin, A. S., Dockrell, H. S., Gotten Wiker, H., Ulvund, G., Holm, A., Jorgensen, M. C., Andersen, P. (1998). B-Cell Epitopes and Quantification of the ESAT-6 Protein of Mycobacterium tuberculosis. Infect. Immun. 66: 717-723 [Abstract] [Full Text]  
• Theisen, M., Soe, S., Oeuvray, C., Thomas, A. W., Vuust, J., Danielsen, S., Jepsen, S., Druilhe, P. (1998). The Glutamate-Rich Protein (GLURP) of Plasmodium falciparum Is a Target for Antibody-Dependent Monocyte-Mediated Inhibition of Parasite Growth In Vitro. Infect. Immun. 66: 11-17 [Abstract] [Full Text]