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Clinical and Vaccine Immunology, March 2008, p. 468-473, Vol. 15, No. 3
1071-412X/08/$08.00+0     doi:10.1128/CVI.00467-07
Copyright © 2008, American Society for Microbiology. All Rights Reserved.

Species-Specific Antibody Responses to the Recombinant 53-Kilodalton Excretory and Secretory Proteins in Mice Infected with Trichinella spp.{triangledown}

Isao Nagano,* Zhiliang Wu, and Yuzo Takahashi

Department of Parasitology, Gifu University Graduate School of Medicine, Yanagido 1-1, Gifu, 501-1194 Japan

Received 26 November 2007/ Returned for modification 15 December 2007/ Accepted 29 December 2007

The 53-kDa proteins in larval excretory and secretory (E-S) products were expressed from five Trichinella species (T. spiralis, T. britovi, T. nativa, T. pseudospiralis, and T. papuae), using the Escherichia coli expression system, and the antibody responses to the 53-kDa recombinant proteins in mice infected with Trichinella spp. were analyzed by Western blotting. The 53-kDa protein is conserved among the five Trichinella species, with >60% similarity in amino acid sequences. The 53-kDa recombinant proteins of T. spiralis and T. pseudospiralis reacted to sera from mice infected with T. spiralis and T. pseudospiralis at 8 days postinfection (p.i.), respectively. An antibody against the 53-kDa recombinant protein of T. spiralis recognized the 53-kDa protein in the crude extracts from adult worms and 30-day p.i. muscle larvae and E-S products from muscle larvae of T. spiralis but did not recognize any proteins from T. pseudospiralis. The sera from the mice infected with T. spiralis strongly reacted with the 53-kDa recombinant protein of T. spiralis but did not react with the 53-kDa recombinant proteins of T. britovi, T. nativa, T. pseudospiralis, and T. papuae. Similarly, the sera from mice infected with T. britovi, T. nativa, T. pseudospiralis, or T. papuae strongly reacted with the 53-kDa recombinant proteins of T. britovi, T. nativa, T. pseudospiralis, or T. papuae, respectively. These results showed that the 53-kDa recombinant proteins provide early and species-specific antibody responses in mice infected with Trichinella spp.

* Corresponding author. Mailing address: Department of Parasitology, Gifu University Graduate School of Medicine, Yanagido 1-1 Gifu, 501-1194 Japan. Phone: 81 58 230 6367. Fax: 81 58 230 6368. E-mail: isao{at}gifu-u.ac.jp

{triangledown} Published ahead of print on 9 January 2008.

Clinical and Vaccine Immunology, March 2008, p. 468-473, Vol. 15, No. 3
1071-412X/08/$08.00+0     doi:10.1128/CVI.00467-07
Copyright © 2008, American Society for Microbiology. All Rights Reserved.





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