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Clinical and Vaccine Immunology, March 2008, p. 439-446, Vol. 15, No. 3
1071-412X/08/$08.00+0     doi:10.1128/CVI.00208-07
Copyright © 2008, American Society for Microbiology. All Rights Reserved.

Primary and Secondary Infections of Macaca fascicularis Monkeys with Asian and American Genotypes of Dengue Virus 2{triangledown}

Lidice Bernardo,1 Alienys Izquierdo,1 Irina Prado,1 Delfina Rosario,1 Mayling Alvarez,1 Emidalys Santana,1 Jorge Castro,2 Rafael Martínez,2 Rosmari Rodríguez,1 Luis Morier,1 Gerardo Guillén,2 and María G. Guzmán1*

Department of Virology, PAHO/WHO Collaborating Center for the Study of Dengue and its Vector, Pedro Kourí Tropical Medicine Institute, Autopista Novia del Mediodía, km 6 1/2, P.O. Box 601, Marianao 13, Havana, Cuba,1 Center for Genetic Engineering and Biotechnology, Apdo 6162, Havana 10600, Cuba2

Received 11 May 2007/ Returned for modification 5 November 2007/ Accepted 6 December 2007

The goal of this study was to compare the immune response and the protection capacity induced by the dengue virus 2 (DENV-2) American and Asian genotypes in Macaca fascicularis monkeys. Animals were infected with American or Asian DENV-2 strains and challenged 1 year later with a DENV-2 Asian genotype strain. The viremia and monkey antibody levels were similar for the different strains after primary and secondary infection; however, the functionality of the antibody response was different. A limited viral replication was demonstrated after the secondary infection in all the monkeys. No virus was isolated in tissue culture, while reverse transcription-PCR showed a late positive reaction in four of five challenged monkeys. The immunoglobulin M response pattern and the detection of antibodies to specific proteins by Western blotting supported the protection data. Despite the demonstration of the protective effect after homologous challenge, a strong anamnestic antibody response was observed.

* Corresponding author. Mailing address: Department of Virology, Pedro Kourí Tropical Medicine Institute (IPK), Autopista Novia del Mediodía, Km 6 1/2, P.O. Box 601, Marianao 13, Havana, Cuba. Phone: 53-7-2020450. Fax: 53-7-2046051. E-mail: lupe{at}ipk.sld.cu

{triangledown} Published ahead of print on 19 December 2007.

Clinical and Vaccine Immunology, March 2008, p. 439-446, Vol. 15, No. 3
1071-412X/08/$08.00+0     doi:10.1128/CVI.00208-07
Copyright © 2008, American Society for Microbiology. All Rights Reserved.





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