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Clinical and Vaccine Immunology, January 2008, p. 95-100, Vol. 15, No. 1
1071-412X/08/$08.00+0     doi:10.1128/CVI.00338-07
Copyright © 2008, American Society for Microbiology. All Rights Reserved.

Leucine Aminopeptidase Is an Immunodominant Antigen of Fasciola hepatica Excretory and Secretory Products in Human Infections{triangledown}

A. Marcilla,1* J. E. De la Rubia,1 J. Sotillo,1 D. Bernal,2 C. Carmona,3 Z. Villavicencio,4 D. Acosta,3 J. Tort,5 F. J. Bornay,4 J. G. Esteban,1 and R. Toledo1

Área de Parasitología, Departamento de Biología Celular y Parasitología, Facultad de Farmacia, Universitat de València, Av. Vicent Andres Estelles s/n, 46100 Burjassot, Valencia, Spain,1 Departamento de Bioquímica y Biología Molecular, Facultat de Ciències Biològiques, Universitat de Valencia, Valencia, Spain,2 Unidad de Biología Parasitaria, Departamento de Biología Celular y Molecular, Facultad de Ciencias, Instituto de Higiene, Av. A. Navarro 3051 CP11600, Montevideo, Uruguay,3 División de Parasitología, Facultad de Farmacia, Universidad Miguel Hernández, Alicante, Spain,4 Departamento de Genética, Facultad de Medicina, Universidad de la República, Av. Gral. Flores 2121 CP11800, Montevideo, Uruguay5

Received 7 August 2007/ Returned for modification 2 October 2007/ Accepted 2 November 2007

The liver fluke Fasciola hepatica parasitizes humans and ruminant livestock worldwide, and it is now being considered a reemerging zoonotic disease, especially in areas in which it is endemic, such as South America. This study investigates the immune response to excretory and secretory products produced by F. hepatica in a group of patients from the Peruvian Altiplano, where the disease is highly endemic. Using a proteomic approach and immunoblotting techniques, we have identified the enzymes leucine aminopeptidase (LAP) and phosphoenolpyruvate carboxykinase as immunodominant antigens recognized by sera from fasciolosis patients. An indirect enzyme-linked immunosorbent assay using recombinant LAP as the antigen was developed to check sera from individuals of this region. Our results demonstrate that LAP produces a specific and strong reaction, suggesting its potential use in the serologic diagnosis of F. hepatica infections in humans.

* Corresponding author. Mailing address: Departamento Biologia Celular y Parasitologia, Facultat de Farmàcia, Universitat de València, Av. V.A. Estellés s/n, 46100 Burjassot, Valencia, Spain. Phone: 34-963544491. Fax: 34-963544769. E-mail: Antonio.Marcilla{at}uv.es

{triangledown} Published ahead of print on 14 November 2007.

Clinical and Vaccine Immunology, January 2008, p. 95-100, Vol. 15, No. 1
1071-412X/08/$08.00+0     doi:10.1128/CVI.00338-07
Copyright © 2008, American Society for Microbiology. All Rights Reserved.





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