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Clinical and Vaccine Immunology, April 2007, p. 481-483, Vol. 14, No. 4
1071-412X/07/$08.00+0     doi:10.1128/CVI.00402-06
Copyright © 2007, American Society for Microbiology. All Rights Reserved.

Evaluation of Alloreactivity in Responder-Stimulator Pairs by Determination of Gamma Interferon-Producing Cells and Cytotoxic-T-Lymphocyte Precursor Frequencies

Transplantation Immunology, San Giovanni Battista Hospital, Turin, and Department of Genetics, Biology and Biochemistry, University of Turin, Turin, Italy

Received 28 October 2006/ Returned for modification 5 January 2007/ Accepted 7 February 2007

We used the enzyme-linked immunospot (ELISPOT) assay and the cytotoxic-T-lymphocyte precursor frequency assay to evaluate alloreactivity in responder-stimulator pairs. High frequencies of responder cells among cells from HLA-mismatched pairs and low frequencies among cells from pairs of siblings with identical HLA types were detected by both assays. The ELISPOT assay thus illustrated the helper and cytotoxic-T-cell response to allogeneic HLA antigens.

Published ahead of print on 14 February 2007.