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Clinical and Vaccine Immunology, March 2007, p. 312-317, Vol. 14, No. 3
1071-412X/07/$08.00+0     doi:10.1128/CVI.00353-06
Copyright © 2007, American Society for Microbiology. All Rights Reserved.

Production and Characterization of Monoclonal Antibodies against a Major Membrane Protein of Mycobacterium avium subsp. paratuberculosis{triangledown}

John P. Bannantine,1* Thomas J. Radosevich,1 Judith R. Stabel,1 Sven Berger,2 J. Frank T. Griffin,2 and Michael L. Paustian1

National Animal Disease Center, USDA-ARS, Ames, Iowa 50010,1 Disease Research Laboratory, Department of Microbiology and Immunology, University of Otago, Dunedin, New Zealand2

Received 28 September 2006/ Returned for modification 11 December 2006/ Accepted 20 January 2007

The Mycobacterium avium subsp. paratuberculosis 35-kDa major membrane protein (MMP) encoded by MAP2121c is an important membrane antigen recognized in cattle with Johne's disease. In this study, purified recombinant MMP was used to produce two stable monoclonal antibodies, termed 8G2 and 13E1, which were characterized by immunoblotting, epitope mapping, and immunofluorescence microscopy.

* Corresponding author. Mailing address: National Animal Disease Center, ARS-USDA, 2300 North Dayton Ave., Ames, IA 50010. Phone: (515) 663-7340. Fax: (515) 663-7458. E-mail: jbannant{at}nadc.ars.usda.gov.

{triangledown} Published ahead of print on 31 January 2007.

Clinical and Vaccine Immunology, March 2007, p. 312-317, Vol. 14, No. 3
1071-412X/07/$08.00+0     doi:10.1128/CVI.00353-06
Copyright © 2007, American Society for Microbiology. All Rights Reserved.



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