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Clinical and Vaccine Immunology, March 2007, p. 304-311, Vol. 14, No. 3
1071-412X/07/$08.00+0     doi:10.1128/CVI.00363-06
Copyright © 2007, American Society for Microbiology. All Rights Reserved.

DNA Vaccine Construct Incorporating Intercellular Trafficking and Intracellular Targeting Motifs Effectively Primes and Induces Memory B- and T-Cell Responses in Outbred Animals

Veterinary Microbiology and Pathology, Washington State University, Pullman, Washington 99164,¹ Animal Health & Biomedical Sciences, University of Wisconsin—Madison, Madison, Wisconsin 53706,² Institute of Animal Health, Compton, United Kingdom,³ Retrovirus Research Unit, RIKEN, Wako, Saitama 351-0198, Japan⁴

Received 4 October 2006/ Returned for modification 8 December 2006/ Accepted 28 December 2006

We developed a vaccine construct in which a BVP22 domain and an invariant-chain major histocompatibility complex class II-targeting motif capable of enhancing dendritic cell antigen uptake and presentation were fused to a sequence encoding a B- and T-cell antigen from the Anaplasma marginale major surface protein 1a and tested whether this construct would prime and expand immune responses in outbred calves. A single inoculation with this construct effectively primed the immune responses, as demonstrated by a significant enhancement of CD4⁺ T-cell proliferation compared to that in calves identically inoculated but inoculated with a DNA construct lacking the targeting domains and compared to that in calves inoculated with an empty vector. These proliferative responses were mirrored by priming and expansion of gamma interferon-positive CD4⁺ T cells and immunoglobulin G responses against the linked B-cell epitope. Priming by the single immunization induced memory that underwent rapid recall following reexposure to the antigen. These results demonstrate that DNA vaccines targeting key intercellular and intracellular events significantly enhance priming and expansion and support the feasibility of single-dose DNA immunization in outbred populations.

Published ahead of print on 10 January 2007.

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