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Clinical and Vaccine Immunology, December 2007, p. 1646-1648, Vol. 14, No. 12
1071-412X/07/$08.00+0     doi:10.1128/CVI.00293-07
Copyright © 2007, American Society for Microbiology. All Rights Reserved.

Western Blot Assay Using Recombinant p26 Antigen for Detection of Equine Infectious Anemia Virus-Specific Antibodies{triangledown}

I. Alvarez,1* G. Gutierrez,1 E. Ostlund,2 M. Barrandeguy,1 and K. Trono1

Instituto de Virología, Centro de Investigaciones en Ciencias Veterinarias y Agronómicas, Instituto Nacional de Tecnología Agropecuaria, C.C. 1712, Castelar, Argentina,1 National Veterinary Services Laboratories, P.O. Box 844, Ames, Iowa 500102

Received 17 July 2007/ Returned for modification 18 September 2007/ Accepted 16 October 2007

We analyzed the performance of a single-band Western blot (WB) test using recombinant p26 (rp26) capsid protein of equine infectious anemia virus. According to the results obtained, the rp26 WB test is a reliable confirmatory diagnostic tool to be used as a complementary test after an enzyme-linked immunosorbent assay or agar gel immunodiffusion test yielding doubtful results.

* Corresponding author. Mailing address: Instituto de Virología, Centro de Investigaciones en Ciencias Veterinarias y Agronómicas, INTA, C.C. 1712, Castelar, Argentina. Phone and fax: 54 11 4621 9050. E-mail: ialvarez{at}cnia.inta.gov.ar

{triangledown} Published ahead of print on 24 October 2007.

Clinical and Vaccine Immunology, December 2007, p. 1646-1648, Vol. 14, No. 12
1071-412X/07/$08.00+0     doi:10.1128/CVI.00293-07
Copyright © 2007, American Society for Microbiology. All Rights Reserved.





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Copyright © 2007 by the American Society for Microbiology. All rights reserved.