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Clinical and Vaccine Immunology, September 2006, p. 1037-1043, Vol. 13, No. 9
1071-412X/06/$08.00+0     doi:10.1128/CVI.00110-06
Copyright © 2006, American Society for Microbiology. All Rights Reserved.

Stimulation of Human Monocytes with the Gram-Positive Vaccine Vector Streptococcus gordonii

Annalisa Ciabattini, Anna Maria Cuppone, Rita Pulimeno, Francesco Iannelli, Gianni Pozzi, and Donata Medaglini*

Laboratorio di Microbiologia Molecolare e Biotecnologia, Dipartimento di Biologia Molecolare, Università di Siena, 53100 Siena, Italy

Received 20 March 2006/ Returned for modification 23 May 2006/ Accepted 7 July 2006

Streptococcus gordonii is a bacterial vaccine vector which has previously been shown to activate dendritic cells in vitro and to induce local and systemic immune responses in vivo. In the present study, human monocytes (THP-1 cell line and peripheral blood monocytes) were characterized following interaction with S. gordonii. Treatment of human monocytes with S. gordonii but not latex beads induced a clear up-regulation of CD83, CD40, CD80, and CD54 and the down-regulation of CD14. Furthermore, bacterial treatment stimulated an increased expression of Toll-like receptor 5 (TLR5), TLR6, and TLR7, production of the proinflammatory cytokines tumor necrosis factor alpha and interleukin 1 beta, and reduction of the phagocytic activity. This work shows that the immunostimulatory activity of S. gordonii is not restricted to induction of dendritic-cell maturation but also affects the differentiation process of human monocytes.

* Corresponding author. Mailing address: Laboratorio di Microbiologia Molecolare e Biotecnologia (LA.M.M.B.), Dipartimento di Biologia Molecolare, Università di Siena, Policlinico Le Scotte, Viale Bracci, 53100 Siena, Italy. Phone: 39 0577 233307. Fax: 39 0577 233334. E-mail: medaglini{at}unisi.it.

Clinical and Vaccine Immunology, September 2006, p. 1037-1043, Vol. 13, No. 9
1071-412X/06/$08.00+0     doi:10.1128/CVI.00110-06
Copyright © 2006, American Society for Microbiology. All Rights Reserved.



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