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Clinical and Vaccine Immunology, July 2006, p. 814-817, Vol. 13, No. 7
1071-412X/06/$08.00+0     doi:10.1128/CVI.00035-06
Copyright © 2006, American Society for Microbiology. All Rights Reserved.

Epitope-Blocking Enzyme-Linked Immunosorbent Assay for Detection of Antibodies to Ross River Virus in Vertebrate Sera

Nidia M. M. Oliveira,1,{dagger} Annette K. Broom,1 John S. Mackenzie,2,{ddagger} David W. Smith,3 Michael D. A. Lindsay,1,4 Brian H. Kay,5 and Roy A. Hall2*

Arbovirus Surveillance and Research Laboratory, Department of Microbiology, The University of Western Australia, Nedlands, Western Australia,1 School of Molecular and Microbial Sciences, The University of Queensland, Brisbane, Queensland,2 Division of Health Sciences, The Western Australian Centre for Pathology and Research, Queen Elizabeth II Medical Centre, Nedlands, Western Australia,3 Mosquito-Borne Disease Control Branch, Western Australian Department of Health, Perth, Western Australia,4 Queensland Institute of Medical Research and Australian Centre for International and Tropical Health and Nutrition, Brisbane, Queensland, Australia5

Received 27 January 2006/ Returned for modification 6 March 2006/ Accepted 5 May 2006

We describe the development of an epitope-blocking enzyme-linked immunosorbent assay (ELISA) for the sensitive and rapid detection of antibodies to Ross River virus (RRV) in human sera and known vertebrate host species. This ELISA provides an alternative method for the serodiagnosis of RRV infections.

* Corresponding author. Mailing address: School of Molecular and Microbial Sciences, The University of Queensland, Brisbane, Qld. 4072, Australia. Phone: 61 7 33654647. Fax: 61 7 33654620. E-mail: roy.hall{at}uq.edu.au.

{dagger} Present address: Laboratory of Molecular Virology, National Institute of Mental Health, Room 5C-20, 49 Convent Drive, Bethesda, MD 20892.

{ddagger} Present address: Australian Biosecurity CRC, Curtin University of Technology, GPO Box U1987, Perth, WA 6845, Australia.

Clinical and Vaccine Immunology, July 2006, p. 814-817, Vol. 13, No. 7
1071-412X/06/$08.00+0     doi:10.1128/CVI.00035-06
Copyright © 2006, American Society for Microbiology. All Rights Reserved.





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Copyright © 2006 by the American Society for Microbiology. All rights reserved.