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Clinical and Vaccine Immunology, May 2006, p. 598-601, Vol. 13, No. 5
1071-412X/06/$08.00+0     doi:10.1128/CVI.13.5.598-601.2006
Copyright © 2006, American Society for Microbiology. All Rights Reserved.

A Simple Manual Rosetting Method for Absolute CD4+ Lymphocyte Counting in Resource-Limited Countries

Phonethipsavanh Nouanthong,1,2 Supansa Pata,1 Thira Sirisanthana,2 and Watchara Kasinrerk1,3*

Division of Clinical Immunology, Department of Medical Technology, Faculty of Associated Medical Sciences, Chiang Mai University, Chiang Mai 50200, Thailand,1 Research Institute for Health Sciences, Chiang Mai University, Chiang Mai 50200, Thailand,2 Medical Biotechnology Unit, National Center for Genetic Engineering and Biotechnology, National Science and Technology Development Agency at the Faculty of Associated Medical Sciences, Chiang Mai University, Chiang Mai 50200, Thailand3

Received 25 December 2005/ Returned for modification 6 February 2006/ Accepted 6 March 2006

A CD4 monoclonal antibody which reacts with CD4+ lymphocytes but does not react significantly with monocytes was generated and used to develop a simple method for CD4 count. In a comparison with standard flow cytometry, high correlation was obtained. The developed method can be an alternative to flow cytometry for resource-limited countries.

* Corresponding author. Mailing address: Division of Clinical Immunology, Department of Medical Technology, Faculty of Associated Medical Sciences, Chiang Mai University, Chiang Mai 50200, Thailand. Phone: 6653 945070. Fax: 6653 946043. E-mail: watchara{at}chiangmai.ac.th.

Clinical and Vaccine Immunology, May 2006, p. 598-601, Vol. 13, No. 5
1071-412X/06/$08.00+0     doi:10.1128/CVI.13.5.598-601.2006
Copyright © 2006, American Society for Microbiology. All Rights Reserved.



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