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Clinical and Vaccine Immunology, November 2006, p. 1294-1295, Vol. 13, No. 11
1071-412X/06/$08.00+0     doi:10.1128/CVI.00218-06
Copyright © 2006, American Society for Microbiology. All Rights Reserved.

Dark Immunofluorescence: Correlation with Serum Immunoglobulin Abnormalities{triangledown}

J. List,1* M. S. Buckland,2 B. Thobhani,1 C. J. Sheed,1 J. C. Mann,3 M. Claxton,3 and B. Heelan2

Department of Haematology (Immunology Section),1 Department of Clinical Biochemistry, Colchester General Hospital, Turner Road, Colchester, Essex CO4 5JL,3 Department of Immunology, St. Bartholomew's Hospital, London EC1A 7BE, United Kingdom2

Received 10 June 2006/ Returned for modification 25 July 2006/ Accepted 29 August 2006

Occasional serum samples (<0.5%) tested by indirect immunofluorescence showed less fluorescence than did negative-control serum. A retrospective review of these patients' serum immunoglobulins revealed a high percentage of abnormalities (71%, versus 22% of controls). We suggest that this observation should be reported when seen and that the clinician should be alerted to an association with immunoglobulin abnormalities.

* Corresponding author. Mailing address: Department of Haematology (Immunology), Colchester General Hospital, Turner Road, Colchester, Essex CO4 5JL, United Kingdom. Phone: 01206 742406. Fax: 01206 742400. E-mail: jenny.list{at}essexrivers.nhs.uk.

{triangledown} Published ahead of print on 13 September 2006.

Clinical and Vaccine Immunology, November 2006, p. 1294-1295, Vol. 13, No. 11
1071-412X/06/$08.00+0     doi:10.1128/CVI.00218-06
Copyright © 2006, American Society for Microbiology. All Rights Reserved.





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