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Clinical and Vaccine Immunology, November 2006, p. 1197-1203, Vol. 13, No. 11
1071-412X/06/$08.00+0     doi:10.1128/CVI.00174-06
Copyright © 2006, American Society for Microbiology. All Rights Reserved.

Measurement of Plasma-Derived Substance P: Biological, Methodological, and Statistical Considerations{triangledown}

Donald E. Campbell,1* Nancy Raftery,1 Richard Tustin III,1 Nancy B. Tustin,1 Michelle L. DeSilvio,2 Avital Cnaan,2 Pyone Pyone Aye,3 Andrew A. Lackner,3 and Steven D. Douglas1

Division of Allergy and Immunology,1 Division of Biostatistics and Epidemiology, Joseph Stokes Jr. Research Institute at The Children's Hospital of Philadelphia and Department of Pediatrics, University of Pennsylvania School of Medicine, Philadelphia, Pennsylvania,2 Tulane National Primate Research Center, Covington, Louisiana3

Received 11 May 2006/ Returned for modification 20 June 2006/ Accepted 9 August 2006

The undecapeptide substance P (SP) is a member of the tachykinin family of neurotransmitters, which has a pivotal role in the regulation of inflammatory and immune responses. One of the major barriers to the study of the in vivo role of SP in a number of immune disorders is the accurate measurement of SP in fluids. This is reflected in the variability of reported SP levels in serum and plasma of humans in both healthy and diseased states. This study was initiated in order to identify sources of variability by the comparative evaluation of the influences of sample preparation and analytical detection methods on the measurement of SP in plasma. The results indicate that sample preparation (peptide extraction versus no extraction) and the choice of analytical method for SP quantitation may yield significantly different values and may contribute to the variability in SP values reported in the literature. These results further emphasize the need for careful consideration in the selection of methods for SP quantitation, as well as caution in the interpretation and comparison of data reported in the literature.

* Corresponding author. Mailing address: Division of Allergy and Immunology, The Children's Hospital of Philadelphia, 34th and Civic Center Blvd., Philadelphia, PA 19104. Phone: (215) 590-2353. Fax: (215) 590-3044. E-mail: campbelld{at}email.chop.edu.

{triangledown} Published ahead of print on 13 September 2006.

Clinical and Vaccine Immunology, November 2006, p. 1197-1203, Vol. 13, No. 11
1071-412X/06/$08.00+0     doi:10.1128/CVI.00174-06
Copyright © 2006, American Society for Microbiology. All Rights Reserved.





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