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Detection of Aerosolized Alternaria alternata Conidia, Hyphae, and Fragments by Using a Novel Double-Immunostaining Technique

Department of Medicine, The University of Sydney, NSW, Australia,¹ Woolcock Institute of Medical Research, Sydney, NSW, Australia,² Health Effects Laboratory Division, Centers for Disease Control and Prevention, National Institute of Occupational Safety and Health, Morgantown, West Virginia³

Received 30 March 2005/ Returned for modification 15 June 2005/ Accepted 22 June 2005

A double-immunostaining halogen immunoassay was developed to identify aerosolized conidia, hyphae, and fragments of Alternaria alternata by using an anti-Alternaria polyclonal antiserum, while, simultaneously, allergy to these components was concurrently determined by using human immunoglobulin E antibodies.