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Clinical and Diagnostic Laboratory Immunology, July 2005, p. 882-884, Vol. 12, No. 7
1071-412X/05/$08.00+0     doi:10.1128/CDLI.12.7.882-884.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.

Immunoglobulin M Enzyme-Linked Immunosorbent Assay Using Recombinant Polypeptides for Diagnosis of Dengue

Elsa Videa,1 Maria Josefina Coloma,2 Flavia Barreto dos Santos,3 Angel Balmaseda,1 and Eva Harris2*

Virology Laboratory, Centro Nacional de Diagnóstico y Referencia, Ministry of Health, Managua, Nicaragua,1 Division of Infectious Diseases, School of Public Health, University of California, Berkeley, California,2 Flavivirus Laboratory, Department of Virology, Oswaldo Cruz Institute, Rio de Janeiro, Brazil3

Received 19 January 2005/ Returned for modification 4 April 2005/ Accepted 19 April 2005

We demonstrate that a mixture of four recombinant dengue virus E polypeptides corresponding to the N-terminal region of the envelope protein from all serotypes substitutes for standard antigens in two immunoglobulin M enzyme-linked immunosorbent assay formats with 100% concordance, making these polypeptides a useful and accessible reagent for serological diagnosis of dengue in endemic countries.

* Corresponding author. Mailing address: Division of Infectious Diseases, School of Public Health, 140 Warren Hall, University of California, Berkeley, CA 94720-7360. Phone: (510) 642-4845. Fax: (510) 642-6350. E-mail: eharris{at}socrates.berkeley.edu.

Clinical and Diagnostic Laboratory Immunology, July 2005, p. 882-884, Vol. 12, No. 7
1071-412X/05/$08.00+0     doi:10.1128/CDLI.12.7.882-884.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.





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Antimicrob. Agents Chemother. Clin. Microbiol. Rev. Infect. Immun.
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Copyright © 2005 by the American Society for Microbiology. All rights reserved.