CVI
Home Help [Feedback] [For Subscribers] [Archive] [Search] [Contents]
This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Granade, T. C.
Right arrow Articles by Tancho, S.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Granade, T. C.
Right arrow Articles by Tancho, S.
Clinical and Diagnostic Laboratory Immunology, July 2005, p. 855-860, Vol. 12, No. 7
1071-412X/05/$08.00+0     doi:10.1128/CDLI.12.7.855-860.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.

Evaluation of Rapid Prenatal Human Immunodeficiency Virus Testing in Rural Cameroon

Timothy C. Granade,1* Bharat S. Parekh,1 Pius M. Tih,2 Thomas Welty,2 Edith Welty,2 Marc Bulterys,3 George Ndikintum,2 Godlove Nkuoh,2 and Samuel Tancho2

HIV Laboratory Branch, Division of HIV/AIDS Prevention, National Center for HIV, STD, and TB Prevention,1 Epidemiology Branch, Division of HIV/AIDS Prevention, National Center for HIV, STD and TB Prevention, Centers for Disease Control and Prevention, Atlanta, GA 30333,3 Cameroon Baptist Convention Health Board, P.O. Box 9, Nso, Northwest Province, Cameroon2

Received 3 May 2004/ Returned for modification 24 June 2004/ Accepted 25 April 2005

Pregnant women (n = 859) in rural Cameroonian prenatal clinics were screened by two rapid human immunodeficiency virus (HIV) antibody tests (rapid tests [RT]) (Determine and Hema-Strip) using either whole blood or plasma. One additional RT (Capillus, HIV-CHEK, or Sero-Card) was used to resolve discordant results. RT results were compared with HIV-1 enzyme immunoassay (EIA) and Western blot (WB) results of matched dried blood spots (DBS) to assess the accuracy of HIV RTs. DBS EIA/WB identified 83 HIV antibody-reactive, 763 HIV antibody-nonreactive, and 13 indeterminate specimens. RT results were evaluated in serial (two consecutive tests) or parallel (two simultaneous tests) testing algorithms. A serial algorithm using Determine and Hema-Strip yielded sensitivity and specificity results of 97.6% and 99.7%, respectively, whereas a parallel RT algorithm using Determine plus a second RT produced a sensitivity and specificity of 100% and 99.7%, respectively. HIV RTs provide excellent alternatives for identifying HIV infection, and their field performance could be monitored using DBS testing strategies.

* Corresponding author. Mailing address: Centers for Disease Control and Prevention, 1600 Clifton Rd., NE MS D-12, Atlanta, GA 30333. Phone: (404) 639-3850. Fax: (404) 639-2660. E-mail: Tgranade{at}cdc.gov.

Clinical and Diagnostic Laboratory Immunology, July 2005, p. 855-860, Vol. 12, No. 7
1071-412X/05/$08.00+0     doi:10.1128/CDLI.12.7.855-860.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.





Home Help [Feedback] [For Subscribers] [Archive] [Search] [Contents]
Antimicrob. Agents Chemother. Clin. Microbiol. Rev. Infect. Immun.
J. Clin. Microbiol. J. Virol. ALL ASM JOURNALS

Copyright © 2005 by the American Society for Microbiology. All rights reserved.