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Construction and Expression of Recombinant Streptolysin-O and Preevaluation of Its Use in Immunoassays

Department of Biotechnology, Institute of Hygiene, School of Medicine, Universidad de la República, Uruguay, Av. Alfredo Navarro 3051, Montevideo, Uruguay,¹ Laboratory of Immunotechnology, Department of Immunology, School of Chemistry-School of Science, Universidad de la República, Av. Alfredo Navarro 3051, Montevideo, Uruguay²

Received 22 November 2004/ Returned for modification 3 January 2005/ Accepted 14 March 2005

Commercially available immunoassays for assessment of anti-streptolysin-O antibodies use native streptolysin-O obtained by a complex process. We prepared a biologically active recombinant streptolysin-O with higher yield and a simpler purification process. An enzyme-linked immunosorbent assay developed with this recombinant showed good correlation with a commercial test, suggesting that it could be suitable for immunoassays.