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Detection by Radioligand Assay of Antibodies against Borna Disease Virus in Patients with Various Psychiatric Disorders

Department of Psychiatry, Osaka General Medical Center, Osaka 558-8558,¹ Department of Sleep Disorders Research, Tokyo Institute of Psychiatry, Tokyo Metropolitan Organization for Medical Research, Tokyo 156-8585,² Department of Virology, Research Institute for Microbial Diseases, Osaka University, Osaka 565-0871,³ Research Institute of Bioscience, Azabu University, Kanagawa 229-8501,⁴ Department of Psychiatry, Osaka University Graduate School of Medicine, Osaka 565-0871,⁵ Kuma Hospital, Kobe 650-0011, Japan⁶

Received 15 January 2005/ Returned for modification 7 February 2005/ Accepted 10 March 2005

Using a radioligand assay, which preserves the natural form of the antigen, antibodies against Borna disease virus nucleoprotein and phosphoprotein were detected in 11 and 19 sera of 171 psychiatric patients, respectively. Compared with results by Western blotting, three and nine sera were concordantly positive, respectively. The four sera showing the highest levels of antibodies by radioligand assay were all negative by Western blotting; however, dilution and inhibition tests supported the positive results. Our results suggest the importance of conformational structure to detect human anti-Borna disease virus antibodies.