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Evaluation of a West Nile Virus Immunoglobulin A Capture Enzyme-Linked Immunosorbent Assay

Focus Technologies, Cypress, California

Received 20 August 2004/ Returned for modification 27 September 2004/ Accepted 30 September 2004

An in-house-developed enzyme-linked immunosorbent assay detected West Nile virus (WNV) immunoglobulin A (IgA) in 65 of 68 sera from WNV-infected patients; 40 of 63 WNV IgM-positive, IgG-negative serum or plasma specimens; 65 of 67 WNV IgM-positive, IgG-positive specimens; 0 of 70 WNV IgM-negative, IgG-negative specimens; and 0 of 64 archived blood donation sera. WNV IgA is thus highly prevalent among WNV-infected patients and typically appears after WNV IgM but before WNV IgG.

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