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Fast Dipstick Dye Immunoassay for Detection of Immunoglobulin G (IgG) and IgM Antibodies of Human Toxoplasmosis

Institute of Medical Molecular Biology, Nanjing Medical University, Nanjing,¹ The Key Lab on Molecular Biology of Parasites, Jiangsu Institute of Parasitic Diseases, Wuxi, Jiangsu,² Tongji Medical College, Huazhong University of Science and Technology, Wuhan, Hubei, People's Republic of China³

Received 5 August 2004/ Returned for modification 8 September 2004/ Accepted 13 September 2004

A dipstick dye immunoassay (DDIA) was developed to detect immunoglobulin G (IgG) or IgM antibodies of toxoplasmosis infection in humans. The assays employ a blue colloidal dye particles (D-1) conjugated to sheep anti-human IgG and rabbit anti-human IgM as the visualizing agents and a soluble antigen of tachyzoites of Toxoplasma gondii strain RH (TSA) as the detective antigen. The mixture of dye-labeled anti-human antibody-special human antibody was captured by TSA onto a nitrocellulose membrane dipstick by means of immunochromatography. The assays are rapid (the whole test can be completed within 15 min), simple, and cheap, and they don't require any equipment. They are sensitive and specific for the detection of anti-Toxoplasma IgG or IgM antibodies and generally agree closely with the results from the enzyme-linked immunosorbent assay. The assays are especially suitable for field applications.