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Identification of Another B-Cell Epitope in the Type-Specific Region of Equine Herpesvirus 4 Glycoprotein G

Department of Veterinary Microbiology, Faculty of Agriculture, Yamaguchi University, Yoshida, Yamaguchi,¹ Epizootic Research Station, Equine Research Institute, Japan Racing Association, Shiba, Kokubunji-Machi, Shimotsuga-gun, Tochigi, Japan²

Received 19 July 2004/ Returned for modification 28 September 2004/ Accepted 26 October 2004

Recently, a novel 12-mer B-cell epitope, MKNNPIYSEGSL, in the type-specific region of equine herpesvirus 1 (EHV-1) glycoprotein G (gG) was identified and used as an antigen for enzyme-linked immunosorbent assay (Maeda et al., J. Clin. Microbiol. 42:1095-1098, 2004). Although our prototype strain, TH20p, possesses two repeat sequences containing the B-cell epitope, the EHV-4 NS80567 strain has two repeat sequences that are not identical. One repeat sequence stretch contained the B-cell epitope, while the other contained the 11-mer, MKNNPVYSESL (underlining indicates a different amino acid). In this study, heterogeneity of the type-specific region was compared among Japanese EHV-4 isolates. The 11-mer peptide, MKNNPVYSESL, specifically reacted with sera from horses naturally infected with EHV-4 but not with sera from horses experimentally infected with EHV-4 TH20p. The 11-mer peptide may be another B-cell epitope in the type-specific region.