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Clinical and Diagnostic Laboratory Immunology, November 2004, p. 1060-1063, Vol. 11, No. 6
1071-412X/04/$08.00+0     DOI: 10.1128/CDLI.11.6.1060-1063.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.

Monoclonal Antibodies for Specific Detection of Encephalitozoon cuniculi

Lan Mo and Michel Drancourt*

Unité des Rickettsies, CNRS UMR 6020, Faculté de Médecine, Marseille, France

Received 25 June 2004/ Returned for modification 27 July 2004/ Accepted 2 August 2004

Seven species-specific monoclonal antibodies (MAbs) were produced against Encephalitozoon cuniculi and characterized. The MAbs were immunoglobulin G, and when used for indirect microimmunofluorescence microscopy and Western immunoblot assays, they detected E. cuniculi originating from clinical samples. They did not cross-react with other Encephalitozoon species (E. intestinalis and E. hellem) or with a collection of gram-negative bacteria, yeast, and other parasites. The MAbs reacted primarily with 121-, 56-, 45-, 43-, and 41-kDa protein epitopes of E. cuniculi. These epitopes were demonstrated to be E. cuniculi species specific by sodium dodecyl sulfate-polyacrylamide gel electrophoresis. We developed MAbs to strains of E. cuniculi that can be used successfully to distinguish E. cuniculi from other microsporidial species in cultures established from clinical specimens. These MAbs may provide a specific, simple, rapid, and low-cost tool for the identification and diagnosis of infections due to microsporidia.

* Corresponding author. Mailing address: Unité des Rickettsies, CNRS UMR 6020, Faculté de Médecine, 27 Blvd. Jean Moulin, 13385 Marseille Cedex 5, France. Phone: (33) 4 91 32 43 75. Fax: (33) 4 91 83 03 90. E-mail: Michel.Drancourt{at}medecine.univ-mrs.fr.

Clinical and Diagnostic Laboratory Immunology, November 2004, p. 1060-1063, Vol. 11, No. 6
1071-412X/04/$08.00+0     DOI: 10.1128/CDLI.11.6.1060-1063.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.





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Copyright © 2004 by the American Society for Microbiology. All rights reserved.