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Clinical and Diagnostic Laboratory Immunology, May 2004, p. 615-617, Vol. 11, No. 3
1071-412X/04/$08.00+0     DOI: 10.1128/CDLI.11.3.615-617.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.

Interlaboratory Reliability of Microimmunofluorescence Test for Measurement of Chlamydia pneumoniae-Specific Immunoglobulin A and G Antibody Titers

Alyson J. Littman,1,2* Lisa A. Jackson,1,3 Emily White,1,2 Mark D. Thornquist,2 Charlotte A. Gaydos,4 and Thomas L. Vaughan1,2

Department of Epidemiology, University of Washington,1 Division of Public Health Sciences, Fred Hutchinson Cancer Research Center,2 Center for Health Studies, Group Health Cooperative, Seattle, Washington,3 Division of Infectious Diseases, Johns Hopkins University, Baltimore, Maryland4

Received 17 September 2003/ Returned for modification 29 October 2003/ Accepted 23 January 2004

To evaluate the reliability of Chlamydia pneumoniae-specific immunoglobulin G (IgG) and IgA antibody titers as measured by the microimmunofluorescence (MIF) test, we compared results from 392 individuals using a standard MIF protocol at two academic laboratories. The kappas for dichotomous titers (>=16 versus <16) were 0.39 for IgA and 0.53 for IgG. Measurement error likely attenuates C. pneumoniae-disease associations; the magnitude of attenuation can be estimated from results of studies such as this one.

* Corresponding author. Mailing address: Fred Hutchinson Cancer Research Center, 1100 Fairview Ave. North, M4 B402, Seattle, WA 98109-1024. Phone: (206) 667-7707. Fax: (206) 667-7850. E-mail: alittman{at}fhcrc.org.

Clinical and Diagnostic Laboratory Immunology, May 2004, p. 615-617, Vol. 11, No. 3
1071-412X/04/$08.00+0     DOI: 10.1128/CDLI.11.3.615-617.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.



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Copyright © 2004 by the American Society for Microbiology. All rights reserved.