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Clinical and Diagnostic Laboratory Immunology, January 2004, p. 77-82, Vol. 11, No. 1
1071-412X/04/$08.00+0     DOI: 10.1128/CDLI.11.1.77-82.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.

Calcium Signaling through Phospholipase C Activates Dendritic Cells To Mature and Is Necessary for the Activation and Maturation of Dendritic Cells Induced by Diverse Agonists

Kenneth C. Bagley,1,2 Sayed F. Abdelwahab,1,3 Robert G. Tuskan,1 and George K. Lewis1,3*

Division of Vaccine Research, Institute of Human Virology, University of Maryland Biotechnology Institute,1 Department of Biochemistry and Molecular Biology,2 Department of Microbiology and Immunology, University of Maryland School of Medicine, University of Maryland Baltimore, Baltimore, Maryland 212013

Received 13 June 2003/ Returned for modification 3 October 2003/ Accepted 10 October 2003

Calcium is an important second messenger in the phospholipase C (PLC) signal transduction pathway. Calcium signaling is involved in many biological processes, including muscle contraction, cellular activation, and cellular proliferation. Dendritic cell (DC) maturation is induced by many different stimuli, including bacterial lipopolysaccharide (LPS), bacterial toxins, inflammatory cytokines, prostaglandins, as well as calcium mobilization. In the present study, we determined the role of the PLC signal transduction pathway in the activation and maturation of human monocyte-derived DCs (MDDCs) induced by diverse agonists. We found that signaling through PLC activates MDDCs to mature and is necessary for LPS, cholera toxin, dibutyryl-cyclic AMP, prostaglandin E2, and the calcium ionophore A23187 to induce MDDC maturation. The results of the present study along with the results of other studies indicate that multiple signaling pathways are involved in the activation of DCs and that inhibition of any of these pathways inhibits the maturation of DCs.

* Corresponding author. Mailing address: Division of Vaccine Research, Institute of Human Virology, University of Maryland Baltimore, 725 W. Lombard St., Baltimore, MD 21201. Phone: (410) 706-4688. Fax: (410) 706-4695. E-mail: lewisg{at}umbi.umd.edu.

Clinical and Diagnostic Laboratory Immunology, January 2004, p. 77-82, Vol. 11, No. 1
1071-412X/04/$08.00+0     DOI: 10.1128/CDLI.11.1.77-82.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.



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