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Clinical and Diagnostic Laboratory Immunology, January 2004, p. 168-173, Vol. 11, No. 1
1071-412X/04/$08.00+0     DOI: 10.1128/CDLI.11.1.168-173.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.

Establishment of Adult Peripheral Blood Lymphocyte Subset Reference Range for an Asian Population by Single-Platform Flow Cytometry: Influence of Age, Sex, and Race and Comparison with Other Published Studies

Wee J. Chng,1,2* Guat B. Tan,2 and Ponnudurai Kuperan2

Department of Haematology-Oncology, National University Hospital,1 Department of Pathology and Laboratory Medicine, Tan Tock Seng Hospital, Singapore, Republic of Singapore2

Received 11 August 2003/ Returned for modification 25 September 2003/ Accepted 7 November 2003

We established a normal reference range for peripheral blood lymphocyte subsets in a multiracial adult population by using single-platform flow cytometry. Further analysis of our cohort showed that the CD8+-cell counts decrease with age, there is a gender difference in NK cell percentages and counts, and there are significant differences in the CD3+-, CD4+-, and CD19+-cell counts between Indians and other racial groups. Overall, our results are significantly different from other published data. This difference further stresses the need for different populations to establish their own reference ranges as these may have important implications for the management of patients with human immunodeficiency virus and AIDS. The use of single-platform flow cytometry will eliminate some of the variability between different study centers, making studies more comparable. This platform should be used for future studies into the effects of age, sex, and race on lymphocyte subsets.

* Corresponding author. Mailing address: Department of Haematology-Oncology, National University Hospital, 5 Lower Kent Ridge Rd., Singapore 119074, Republic of Singapore. Phone: (65) 6772 4971. Fax: (65) 6777 5545. E-mail: chngwj{at}nuh.com.sg.

Clinical and Diagnostic Laboratory Immunology, January 2004, p. 168-173, Vol. 11, No. 1
1071-412X/04/$08.00+0     DOI: 10.1128/CDLI.11.1.168-173.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.





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