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Clinical and Diagnostic Laboratory Immunology, November 2003, p. 1136-1140, Vol. 10, No. 6
1071-412X/03/$08.00+0     DOI: 10.1128/CDLI.10.6.1136-1140.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.

Effect of Antigen Coating Conditions on Enzyme-Linked Immunosorbent Assay for Detection of Immunoglobulin G Antibody to Neisseria meningitidis Serogroup Y and W135 Capsular Polysaccharide Antigens in Serum

Peter C. Giardina,* Renee E. Evans, Daniel J. Sikkema, Dace Madore, and Stephen W. Hildreth{dagger}

Department of Applied Immunology and Microbiology, Wyeth Vaccines Research, Rochester, New York 14623

Received 27 May 2003/ Returned for modification 8 July 2003/ Accepted 21 July 2003

Human sera collected from 28 consenting adult volunteers were used to define assay conditions for meningococcal vaccine clinical trial serology. Immunoassay parameters were optimized with these test sera and the standard reference serum, CDC1992. Coating conditions for serogroup Y and W135 polysaccharide antigens were found to influence the predicted serum immunoglobulin G (IgG) antibody concentrations. Sera that displayed IgG antibody binding profiles most unlike that of CDC1992 were influenced the most by coating conditions. Our results suggest that presentation of specific epitopes is influenced by antigen-coating concentrations for serogroup Y and W135 polysaccharides.

* Corresponding author. Mailing address: Department of Applied Immunology and Microbiology, Wyeth Research, 77 Ridgeland Rd., Rochester, NY 14623. Phone: (585) 350-2521. Fax: (585) 350-2552. E-mail: giardip{at}wyeth.com.

{dagger} Present address: Ortho-Clinical Diagnostics, Rochester, NY 14626-5101.

Clinical and Diagnostic Laboratory Immunology, November 2003, p. 1136-1140, Vol. 10, No. 6
1071-412X/03/$08.00+0     DOI: 10.1128/CDLI.10.6.1136-1140.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.



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Copyright © 2003 by the American Society for Microbiology. All rights reserved.