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Clinical and Diagnostic Laboratory Immunology, September 2003, p. 969-972, Vol. 10, No. 5
1071-412X/03/$08.00+0     DOI: 10.1128/CDLI.10.5.969-972.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.

Serotyping Isolates of Anaplasma phagocytophilum by Using Monoclonal Antibodies

Hisashi Inokuma,1,2 Philippe Brouqui,2* J. Stephen Dumler,2,3 and Didier Raoult2

Laboratory of Veterinary Internal Medicine, Faculty of Agriculture, Yamaguchi University, 753-8515 Yamaguchi, Japan,1 Unité des Rickettsies, Faculté de Médecine, Université de la Méditerranée, Marseille Cédex 5, France,2 Department of Pathology, Johns Hopkins University School of Medicine, Baltimore, Maryland 212873

Received 29 January 2003/ Returned for modification 1 May 2003/ Accepted 10 June 2003

Ten mouse monoclonal antibodies (MAbs) that react with Anaplasma phagocytophilum (the human granulocytic ehrlichiosis agent) Webster isolates were developed. Seven different isolates of A. phagocytophilum were subtyped with these MAbs. Western blot analysis revealed that these MAbs reacted mainly with 41- to 46-kDa Msp2 proteins. Six MAbs reacted with all isolates. Four other MAbs reacted with human isolates from Wisconsin, but not with human isolates from New York or with animal isolates. Three different serotypes were identified. These features may lead to the development of other specific MAbs in order to provide tools for antigenic characterization of human isolates of A. phagocytophilum.

* Corresponding author. Mailing address: Unité des Rickettsies, Faculté de Médecine, 27 Bd. Jean Moulin, 13385 Marseille Cédex 5, France. Phone: (33) 4-91-32-43-75. Fax: (33) 4-91-83-03-90. E-mail: Philippe.Brouqui{at}medecine.univ-mrs.fr.

Clinical and Diagnostic Laboratory Immunology, September 2003, p. 969-972, Vol. 10, No. 5
1071-412X/03/$08.00+0     DOI: 10.1128/CDLI.10.5.969-972.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.



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