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Clinical and Diagnostic Laboratory Immunology, September 2003, p. 808-812, Vol. 10, No. 5
1071-412X/03/$08.00+0     DOI: 10.1128/CDLI.10.5.808-812.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.

Effect of Bacterial Flora on Postimmunization Gastritis following Oral Vaccination of Mice with Helicobacter pylori Heat Shock Protein 60

Department of Infectious Disease, Division of Medical Microbiology,¹ First Surgery, Kyorin University School of Medicine, Mitaka-shi,² Department of Microbiology, Leprosy Research Center National Institute of Infectious Diseases, Higashimurayama-shi,³ Jiseikai Hospital, Suginami-ku, Tokyo, Japan⁴

Received 13 January 2003/ Returned for modification 30 April 2003/ Accepted 2 June 2003

In order to assess the efficacy of oral Helicobacter pylori heat shock protein 60 (HSP60) as a vaccine, protection against H. pylori infection in specific-pathogen-free (SPF) C57BL/6 and germfree (GF) IQI mice was examined. Prophylactic oral vaccination of these two strains of mice with either H. pylori HSP60 or Escherichia coli GroEL inhibited H. pylori colonization by 90 to 95% at 3 weeks postinfection (p.i.). However, these mice were only partially protected because bacterial loads increased in all animals at 10 weeks p.i. Anti-H. pylori HSP60 immunoglobulin G was detected in serum at 3 weeks p.i. in mice vaccinated with either H. pylori HSP60 or GroEL. Significant increases in the gastritis scores were observed only in SPF mice immunized with H. pylori HSP60. These results indicate that oral vaccination with H. pylori HSP60 has partial protective effects on subsequent H. pylori infection but also induces postimmunization gastritis. However, GF mice immunized with H. pylori HSP60 did not suffer from severe gastritis. Therefore, the presence of bacterial flora appears to contribute to the induction of postimmunization gastritis.

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