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Development and Evaluation of a Western Blot Kit for Diagnosis of Human Trichinellosis

Laboratoire de Parasitologie-Mycologie, Centre National de Référence des Trichinella Hôpital Cochin, Université R. Descartes, Assistance Publique-Hôpitaux de Paris, 75014 Paris,¹ UMR BIPAR INRA AFSSA ENVA UPVM, Maisons Alfort 94700,² LDBIO Diagnostics, 69009 Lyon, France³

Received 11 February 2003/ Returned for modification 21 March 2003/ Accepted 13 May 2003

We evaluated industrially prepared Western blot strips designed to avoid the cross-reactions observed with indirect immunofluorescence and enzyme-linked immunosorbent assays used for the serodiagnosis of trichinellosis. The antigen preparations were crude extracts of Trichinella spiralis. The Western blot profile characteristic of trichinellosis was characterized by comparing 60 sera from patients infected by Trichinella to 11 sera from healthy subjects, 51 sera from patients with other proven parasitic diseases (cysticercosis, schistosomiasis, strongyloidosis, fascioliasis, toxocariasis, liver amebiasis, anisakiasis, filariasis, toxoplasmosis, hydatidosis, or malaria), and 23 sera from patients with autoantibodies. Specific 43- to 44-kDa and 64-kDa bands were obtained with all of the sera from 51 patients with acute trichinellosis, in 4 out of 9 patients at the early stages of the disease, and in only 1 control patient, who had suspected anisakiasis and in whom trichinellosis could not be ruled out by muscle biopsy.