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Clinical and Diagnostic Laboratory Immunology, May 2003, p. 476-478, Vol. 10, No. 3
1071-412X/03/$08.00+0     DOI: 10.1128/CDLI.10.3.476-478.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.

One-Step Immunochromatographic Dipstick Tests for Rapid Detection of Vibrio cholerae O1 and O139 in Stool Samples

F. Nato,^1* A. Boutonnier,¹ M. Rajerison,² P. Grosjean,² S. Dartevelle,¹ A. Guénolé,¹ N. A. Bhuiyan,³ D. A. Sack,³ G. B. Nair,³ J. M. Fournier,¹ and S. Chanteau^2,

Institut Pasteur, Paris, France,¹ Institut Pasteur, Antananarivo, Madagascar,² Laboratory Sciences Division, International Centre for Diarrhoeal Diseases Research, Bangladesh (ICDDR,B), Dhaka, Bangladesh³

Received 24 October 2002/ Returned for modification 10 December 2002/ Accepted 28 January 2003

We describe the development and evaluation of a rapid diagnostic test for Vibrio cholerae O1 and O139 based on lipopolysaccharide detection using gold particles. The specificity ranged between 84 and 100%. The sensitivity of the dipsticks ranged from 94.2 to 100% when evaluated with stool samples obtained in Madagascar and Bangladesh. The dipstick can provide a simple tool for epidemiological surveys.

Present address: CERMES, Niamey, Niger.

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