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Clinical and Diagnostic Laboratory Immunology, May 2003, p. 352-356, Vol. 10, No. 3
1071-412X/03/$08.00+0     DOI: 10.1128/CDLI.10.3.352-356.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.

Respiratory Disease in Kennelled Dogs: Serological Responses to Bordetella bronchiseptica Lipopolysaccharide Do Not Correlate with Bacterial Isolation or Clinical Respiratory Symptoms

Victoria J. Chalker,* Crista Toomey, Shaun Opperman, Harriet W. Brooks, Michael A. Ibuoye, Joe Brownlie, and Andrew N. Rycroft

Department of Pathology and Infectious Diseases, Royal Veterinary College, University of London, North Mymms, Hertfordshire AL9 7TA, United Kingdom

Received 21 November 2001/ Returned for modification 9 May 2002/ Accepted 27 February 2003

The role of Bordetella bronchiseptica in a natural outbreak of canine infectious respiratory disease was investigated both by culture and serological analysis. B. bronchiseptica was found in the lungs of a large proportion of clinically healthy dogs and in a greater proportion of dogs with respiratory disease. Using a lipopolysaccharide (LPS) antigen-based enzyme-linked immunosorbent assay, we analyzed the serological responses of a large number of dogs. Dogs with high antibody levels showed no protection from disease, and there was no correlation between the development of disease and rising antibody titer. Similarly, there was no difference in antibody levels in dogs with and without B. bronchiseptica in the lungs. Antibodies to LPS have no predictive value in determining which animals will contract respiratory disease, how severe the disease will be, or which dogs will have B. bronchiseptica colonizing the lungs.

* Corresponding author. Mailing address: Department of Pathology & Infectious Diseases, Royal Veterinary College, University of London, Hawkshead Lane, North Mymms, Hertfordshire AL9 7TA, United Kingdom. Phone: 44-(0)1707-666-486. Fax: 44-(0)1707-661-464. E-mail: vchalker{at}rvc.ac.uk.

Clinical and Diagnostic Laboratory Immunology, May 2003, p. 352-356, Vol. 10, No. 3
1071-412X/03/$08.00+0     DOI: 10.1128/CDLI.10.3.352-356.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.



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