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Clinical and Diagnostic Laboratory Immunology, March 2003, p. 241-245, Vol. 10, No. 2
1071-412X/03/$08.00+0     DOI: 10.1128/CDLI.10.2.241-245.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.

Performance of Five Serological Assays for Diagnosis of Rhodococcus equi Pneumonia in Foals

Steeve Giguère,1* Jorge Hernandez,1 Jack Gaskin,2 John F. Prescott,3 Shinji Takai,4 and Corey Miller5

Departments of Large Animal Clinical Sciences,1 Pathobiology, College of Veterinary Medicine, University of Florida, Gainesville, Florida 32610,2 Department of Pathobiology, University of Guelph, Guelph, Ontario, Canada N1G2W1,3 Department of Animal Hygiene, School of Veterinary Medicine and Animal Sciences, Kitasato University, Towada, Aomori 034-8628, Japan,4 Equine Medical Center of Ocala, Ocala, Florida 344745

Received 17 June 2002/ Returned for modification 16 October 2002/ Accepted 16 November 2002

The performance of four enzyme-linked immunosorbent assays (ELISAs) (ELISA-6939, ELISA-33701, ELISA-VapA, and ELISA-California) and an agar gel immunodiffusion test for diagnosis of Rhodococcus equi pneumonia in foals was evaluated. Antibody concentrations of foals with culture-confirmed R. equi pneumonia (n = 41) were compared to those of age-matched pasturemates that remained clinically healthy during the entire breeding season (n = 24). For each serological assay evaluated, selection of a low cutoff resulted in high sensitivity but low specificity. Increasing the cutoff value resulted in better specificity but to the detriment of sensitivity. The best diagnostic performance was achieved with ELISA-California at a cutoff of 40%, resulting in a sensitivity of 59% and a specificity of 88%. We conclude that current serological assays do not differentiate between diseased and clinically healthy foals.

* Corresponding author. Mailing address: Department of Large Animal Clinical Sciences, College of Veterinary Medicine, University of Florida, P.O. Box 100136, 2016 S.W. 16th Ave., Gainesville, FL 32610. Phone: (352) 392-4700, ext. 5678. Fax: (208) 460-3930. E-mail: gigueres{at}mail.vetmed.ufl.edu.

Clinical and Diagnostic Laboratory Immunology, March 2003, p. 241-245, Vol. 10, No. 2
1071-412X/03/$08.00+0     DOI: 10.1128/CDLI.10.2.241-245.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.



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