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Comparison of Serological Assays for Detection of Chlamydia trachomatis Antibodies in Different Groups of Obstetrical and Gynecological Patients

Department of Obstetrics and Gynecology,¹ Department of Medical Microbiology, MCH Westeinde Hospital, The Hague,³ Department of Gynecology, Leiden University Medical Center, Leiden, The Netherlands²

Received 21 February 2002/ Returned for modification 30 May 2002/ Accepted 31 October 2002

New serological enzyme immunoassays (EIAs) were compared with microimmunofluorescence (MIF) as a "gold standard" to detect Chlamydia trachomatis antibodies in different groups of obstetrical, gynecological, and subfertile patients. There were no significant differences in seroprevalence rates, except for the group of C. trachomatis-positive patients (P < 0.01). Test characteristics were calculated for Chlamydia-EIA (Biologische Analysensystem GmbH, Lich, Germany) and pELISA (Medac, Wedel, Germany). pELISA seems to be a good alternative to MIF. It has high specificity and is easier to perform.

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