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Clinical and Diagnostic Laboratory Immunology, January 2003, p. 103-107, Vol. 10, No. 1
1071-412X/03/$08.00+0     DOI: 10.1128/CDLI.10.1.103-107.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.

Characterization of the Humoral Immune Response to Chlamydia Outer Membrane Protein 2 in Chlamydial Infection

I. Portig,1 J. C. Goodall,2 R. L. Bailey,3 and J. S. H. Gaston2*

Department of Medicine, Addenbrooke’s Hospital, Cambridge,2 Department of Clinical Sciences, London School of Hygiene and Tropical Medicine, London, United Kingdom,3 Department of Internal Medicine-Cardiology, Philipps-University, Marburg, Germany1

Received 10 June 2002/ Returned for modification 22 July 2002/ Accepted 17 September 2002

Detection of antibodies to an outer membrane protein 2 (OMP2) by enzyme-linked immunosorbent assay (ELISA) by using either the Chlamydia trachomatis- or the Chlamydia pneumoniae-specific protein was investigated. OMP2 is an immunodominant antigen giving rise to antibody responses in humans infected with different C. trachomatis serovars (A to C and D to K) or with C. pneumoniae, which could be detected by OMP2 ELISA. OMP2 ELISA is not species specific, but antibody titers were usually higher on the homologous protein. The sensitivity of this assay was high but varied according to the "gold standard" applied. Levels of antibody to C. pneumoniae OMP2 as detected by ELISA seem to return to background or near-background values within a shorter period of time compared to antibodies to C. pneumoniae detected by microimmunofluorescence (MIF), making it more likely that positive results in ELISA reflect recent infection. Thus, OMP2 ELISA has distinct advantages over MIF and commercially available ELISAs and might be a useful tool for the serodiagnosis of chlamydial infection.

* Corresponding author. Mailing address: Department of Medicine, Addenbrooke’s Hospital, Cambridge University, Cambridge CB2 ZQQ, United Kingdom. Phone: 44-1223-330 139. Fax: 44-1223-330 160. E-mail: jshg2{at}medschl.cam.ac.uk.

Clinical and Diagnostic Laboratory Immunology, January 2003, p. 103-107, Vol. 10, No. 1
1071-412X/03/$08.00+0     DOI: 10.1128/CDLI.10.1.103-107.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.



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