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Clinical and Diagnostic Laboratory Immunology, 09 1994, 506-510, Vol 1, No. 5
Copyright © 1994 by the American Society for Microbiology. All rights reserved.

Cattle serologically positive for Brucella abortus have antibodies to B. abortus Cu-Zn superoxide dismutase

LB Tabatabai and SG Hennager
National Animal Disease Center, Agricultural Research Service, U.S. Department of Agriculture, Ames, Iowa, USA.

In this study, we demonstrated by a Cu-Zn superoxide dismutase-specific enzyme-linked immunoassay that cattle that are serologically positive for Brucella abortus have serum immunoglobulin G antibodies to B. abortus Cu-Zn superoxide dismutase. The specificity of the antibody reactivity was confirmed by Western blot (immunoblot) analysis with B. abortus salt-extractable proteins containing native Cu-Zn superoxide dismutase and with recombinant B. abortus Cu-Zn superoxide dismutase. The results represent a first step in the direction of the development of a multiprotein diagnostic reagent for bovine brucellosis.


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Antimicrob. Agents Chemother. Clin. Microbiol. Rev. Infect. Immun.
J. Clin. Microbiol. J. Virol. ALL ASM JOURNALS

Copyright © 1994 by the American Society for Microbiology. All rights reserved.