Clinical and Diagnostic Laboratory Immunology, 05 1994, 330-335, Vol 1, No. 3
Copyright © 1994 by the American Society for Microbiology. All rights reserved.

Characterization of substance P binding to human monocytes/macrophages

DR Lucey, JM Novak, VR Polonis, Y Liu and S Gartner
Department of Retroviral Research, Walter Reed Army Institute of Research, Rockville, Maryland 20850, USA.

Substance P (SP), a member of the tachykinin family of neuropeptides, can immunomodulate human T cells and monocytes. SP has been shown to stimulate human monocytes to produce inflammatory cytokines and superoxide ions, and it enhances tumoricidal activity in vitro. A specific SP receptor, however, has not been identified on human monocytes/macrophages. In this study, we report that 125I-SP binds to human monocytes/macrophages with high affinity and specificity (Kd = 2.7 x 10(-8) to 5.5 x 10(-8) M). Our measurements of binding affinity to this single class of receptors were possible only when experiments were performed in the presence of excess serine proteinase inhibitor (serpin) enzyme complex receptor ligand. We determined that 125I-SP bound to a specific receptor on human monocytes/macrophages and that this binding was detectable as early as 6 h and was maintained throughout 6 to 8 weeks in culture. Modulation of the diverse immunological and inflammatory effects of SP on human monocytes may be mediated through this specific SP receptor.

This article has been cited by other articles:

• Lai, J.-P., Douglas, S. D., Wang, Y.-J., Ho, W.-Z. (2005). Real-Time Reverse Transcription-PCR Quantitation of Substance P Receptor (NK-1R) mRNA. CVI 12: 537-541 [Abstract] [Full Text]  
• Arsenescu, R., Blum, A. M., Metwali, A., Elliott, D. E., Weinstock, J. V. (2005). IL-12 Induction of mRNA Encoding Substance P in Murine Macrophages from the Spleen and Sites of Inflammation. J. Immunol. 174: 3906-3911 [Abstract] [Full Text]  
• Lai, J.-P., Ho, W.-Z., Zhan, G.-X., Yi, Y., Collman, R. G., Douglas, S. D. (2001). Substance P antagonist (CP-96,345) inhibits HIV-1 replication in human mononuclear phagocytes. Proc. Natl. Acad. Sci. USA 98: 3970-3975 [Abstract] [Full Text]  
• Marriott, I., Bost, K. L. (2000). IL-4 and IFN-{gamma} Up-Regulate Substance P Receptor Expression in Murine Peritoneal Macrophages. J. Immunol. 165: 182-191 [Abstract] [Full Text]  
• Goode, T., O'Connell, J., Ho, W.-Z., O'Sullivan, G. C., Collins, J. K., Douglas, S. D., Shanahan, F. (2000). Differential Expression of Neurokinin-1 Receptor by Human Mucosal and Peripheral Lymphoid Cells. CVI 7: 371-376 [Abstract] [Full Text]  
• Tripp, R. A., Moore, D., Winter, J., Anderson, L. J. (2000). Respiratory Syncytial Virus Infection and G and/or SH Protein Expression Contribute to Substance P, Which Mediates Inflammation and Enhanced Pulmonary Disease in BALB/c Mice. J. Virol. 74: 1614-1622 [Abstract] [Full Text]  
• Andoh, T., Nagasawa, T., Satoh, M., Kuraishi, Y. (1998). Substance P Induction of Itch-Associated Response Mediated by Cutaneous NK1 Tachykinin Receptors in Mice. J. Pharmacol. Exp. Ther. 286: 1140-1145 [Abstract] [Full Text]